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YOUR ASSIGNMENT THIS WEEK is to take the unknown sequence, go the the NCBI web page, and carry out a BLAST search. When you have located the GenBank record that matches this sequence exactly, go to the features table and locate the positions of the exons under the coding sequence (CDS) portion of the Features table (See “join” followed by nucleotide boundaries for each exon). How do the results of the GeneID and GENSCAN methods compare to the GenBank annotation? Go back to GENSCAN, and redo the analysis, lowering the probability value of correctly identifying exons (the suboptimal exon cutoff) until the initial exon is located. How low did you have to make the p value before the initial codon was located?
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